Methods for controlling intraocular pressure with transition metal complexes

ABSTRACT

Methods for lowering elevated intraocular pressure resulting from the instillation to the eye of sodium hyaluronate during surgery by administration of transition metal complexes are disclosed.

This application is a continuation of application Ser. No. 07/354,582, filed May 22, 1989, now abandoned.

The present invention is directed to transition metal complexes which are useful in controlling intraocular pressure. In particular, the complexes are useful in controlling intraocular pressure resulting from the instillation of exogenous hyaluronate to the eye, particularly during surgery. The present invention is also directed to methods for controlling intraocular pressure resulting from such instillation by the administration of compositions comprising the metal complexes.

According to the present invention, it has been found that metal complexes, particularly transition metal complexes ("TMC") of the following general structure, lower and control intraocular pressure ('IOP") resulting from the instillation of exogenous sodium hyaluronate:

    L.sub.p M.sub.m                                            [A]

wherein L is an organic ligand, M is a transition metal ion and p and m are integers reflecting the nature of the complex, i.e., the ratio of L to M. Examples of preferred TMC are represented by the following structure: ##STR1## wherein:

a is 0 or 1;

b is 1 or 2;

m is Fe⁺³, Fe⁺², Co⁺³, Cr⁺³, V⁺³, Cu⁺² or Mn⁺²

R₁ =R₂ =--OH or --O⁻ ; or

R₁ =--OH or --O⁻ and R₂ =--O--alkyl (C₁ through C₁₈), --O--aryl, --O-alkyl-aryl, --NH--(CH₂)_(n) --NH-peptide (or protein) wherein n=1-6, or --NR₃ R₄, wherein R₃ and/or R₄ can be hydrogen, any saturated or unsaturated alkyl or cycloalkyl group (C₁ through C₁₈), any aryl, alkylaryl, heteroalkyl and heteroaryl group with or without one or more functional groups such as F, Cl, Br or I; amino, imino or nitrilo; alcohol, ether, carbonyl or carboxyl including esters and amides; thiol, thioether, sulfoxide or sulfone; thiocarboxyl and sulfonate including esters and amides; and any phosphorous containing functional group. In addition one or more of any H-atom present in the four acid moieties and/or in the diamine moieties may be substituted by one or more of the following groups: straight chain and/or branched saturated and unsaturated alkyl groups up to 14 carbons; single and/or polynuclear aryl groups up to 14 carbons; alkyl-aryl groups up to 14 carbons; or saturated and/or unsaturated mono or polynuclear heterocyclic groups. Substitution can result in annelation, giving rise to cyclic and polycyclic molecules. These groups which can be substituted for one or more H atoms and which can give rise to cyclic and polycyclic molecules can contain one or more of the following functional groups, in particular: halogen, such as F, Cl Br or I; amino, imino or nitrilo functions; alcohol, ether, carbonyl and carboxyl functions, including esters and amides; thiol, thio ether, sulfoxide; sulfone, thiocarboxylic and sulfonic acid functions; and phosphorus containing functions; z is -1 when R₁ =R₂ =--O⁻ or OH and M is Fe⁺³, Co⁺³, Cr⁺³, or V⁺³ ; z is -2 when R₁ =R₂ =--O⁻ or --OH and M is Fe⁺², Cr⁺² or Mn⁺² and z is O when R₁ is --O⁻ or --OH, R₂ is an ester or an amide and M is Fe⁺³, Co.sup. +3, Cr⁺³ or V⁺³.

The most preferred TMC are the irons (Fe⁺³, Fe⁺²) complexes of the organic ligands.

The TMC of the present invention as described above include all sterioisomers of the TMC.

The TMC of the present invention are formed by reacting the organic ligands with metal ions which are capable of complexing substantially simultaneously with both the nitrogen atoms and the carboxylate groups of the organic ligands, resulting in anionic hexadentates or anionic or neutral tetra-and pentadentates as shown in [B]. The TMC will be anionic or uncharged depending on the oxidation level of the metal ion and the nature of the ligands. Typically the metal ions will be selected from the group consisting of Fe⁺³, Co⁺³, Cr⁺³, V⁺³, Cu⁺² and Mn⁺², preferably Fe⁺³. The synthesis of such TMC is commonly known to those skilled in the art. In general, stoichiometric amounts of the ligand of choice and a water soluble salt of a transition metal such as FeCl₃, Fe(NO)₃ or CuCl₂ are refluxed in water for 5 minutes to an hour. The pH is then adjusted to between 5 and 7 with an appropriate base to provide for the desired salt. Most of the water is then evaporated and the TMC is recrystallized from an alcohol, such as methanol, ethanol, propanol, or alcohol/water mixtures. The formation of Na⁺, NH₄ ⁺ and Ca⁺² salts are described; see for example, Sawyer et al., J. Amer. Chem. Soc., Vol. 81, 816 (1959); Britzinger et al., Z. Anorg. Chem., Vol. 251, 289 (1943) (ref. Beilstein, Vol. 4, III, 1988); and Cohen et al., J. Amer. Chem. Soc., Vol. 88, 3228 (1966).

Organic ligands as described in structure [A] which are reacted with metals to form TMC as described above can be made by several methods. In general, this is accomplished by tetraalkylation of the desired diamine with the appropriate acetate or propionate derivative. The diamino compounds are prepared using standard synthetic methodology familiar to those skilled in the art as described below.

Tetraacetic acid derivatives can be obtained by alkylation of the diamino compound with an excess of an ester of chloro or bromoacetate such as methyl, ethyl, or t-butyl, followed by ester hydrolysis under either acidic (e.g. trifluoroacetic acid or aqueous hydrochloric acid) or basic (e.g. excess sodium or potassium hydroxide or carbonate in aqueous alcohol) conditions. Tetraalkylation can be accomplished in a solvent such as t-butanol in the presence of a base such as sodium or potassium carbonate at 500° C. to reflux for 18 to 48 hours. An alternate method is to use an excess of chloro or bromoacetic acid (for example, see Belcher, R. et. al., Talanta 1959, 3, 201).

Tetrapropionic acid derivatives can be obtained by tetraalkylation using acrylonitrile under forcing conditions followed by hydrolysis of the nitrile functionalities to carboxylic acids using concentrated hydrochloric acid or aqueous sulfuric acid. Alternatively, dialkylation to provide N,N'-dipropionic acids is accomplished by alkylation under milder conditions followed by hydrolysis. In this way, N,N'-dipropionic-N,N'-diacetic acids can be obtained by alkylation of the intermediate diacid with an acetic acid derivative as described above.

N,N'-Diacetic acid-N,N'-di-α-substituted acetic acids can be prepared by initial dialkylation of the diamino compound with an α-halo carboxylic acid (other than a haloacetic acid) to obtain an N,N'-di-α-substituted acetic acid followed by dialkylation with chloro or bromoacetic acid or an ester thereof as described above. (For example, see Nakashima, H.; Miyake, M., Yukagaku 1972, 21, 416).

N,N,N'-Triacetic acid-N'-acetate compounds can be obtained by incomplete hydrolysis of the tetraesters (for example, see Beilstein, Vol. 4, Series III, p.1187).

Monoamide derivatives of ethanediaminetetraacetic acids or propanediaminetetraacetic acids can be prepared by either of two different methods. In the first method, tetraethyl ester derivatives of the diaminetetraacetic acids are hydrolyzed to the triesters using one equivalent of sodium hydroxide in the presence of copper (II) perchlorate. Amide formation is then accomplished with the desired amine and 1,1'-carbonyldiimidazole. The remaining ester functionalities are then hydrolyzed using lithium hydroxide in ethanol (for example, see Hertzberg, R. P.; Dervan, P. B., Biochemistry 1984, 23, 3934). The second procedure involves the formation of the cobalt (III) complex of the diaminetetraacetic acid followed by formation of the amide derivative of the uncoordinated carboxylic acid functionality by using the desired amine and 1-ethyl-(3-dimethylaminopropyl) carbodiimide. The cobalt is then removed from the complex by reduction using iron(II) sulfate and ascorbate at pH 5 to provide the desired monoamide ligand (for example, see Haner, M.; Eidson, A. F.; Darnall, D. W.; Birnbaum, E. R., Arch. Biochem. Biophys. 1984, 231, 477).

Many methods are available for the preparation of substituted ethanediamines and propanediamines. Monosubstituted ethanediamines can be prepared from α-substituted amino acids by conversion of the carboxylic acid functionality to an amide followed by reduction of the amide to an amine (for example, see Yeh, S. M.; Sherman, D. G.; Meares, C. F., Anal. Biochem. 1979, 100, 152). Primary, vicinal diamines can be obtained from a large variety of substituted olefins using several different methods. Olefins can be oxidized to epoxides which are then reacted with azide to provide azide alcohols. Mesylation followed by azide displacement provides a vicinal diazide which can be reduced to the diamine (for example, see Swift, G.; Swern, D., J. Org. Chem. 1967, 32, 511). Or olefins can be oxidized to vicinal diols which can be converted to vicinal diamines by the three step sequence consisting of dimesylation, displacement with azide, and azide reduction (for example, see Martin, R. L.; Norcross, B. E., J. Org. Chem. 1975, 40, 523 or Feit, P. W.; Nielsen, O. T., J. Med. Chem. 1967, 10, 927). Another multistep method is that of Kohn (Kohn, H.; Jung, S-H., J. Am. Chem. Soc. 1983, 103, 4106) which consists of reaction of the olefin with cyanamide and N-bromosuccinimide to provide an alkyl cyanamide that is treated with ethanolic hydrogen chloride to effect hydrolysis to an isourea that is cyclized to an imidazoline using triethylamine or sodium bicarbonate which is then hydrolyzed to the diamine using barium hydroxide. Another method is the reaction of olefins with cyclopentadienylnitrosylcobalt dimer in the presence of nitric oxide followed by reduction with lithium aluminum hydride (see Becker, P. N.; Bergman, R. G., Organomet. 1983, 2, 787).

1,3-Propanediamines with one or two substituents at the 2 position are available by initial alkylation of malonic acid diesters, reduction of the ester functionalities to alcohols using lithium aluminum hydride, conversion of the alcohols to mesylates, mesylate displacement with either azide or phthalimide, and, with azides, reduction or, with phthalimides, hydrolysis to the diamines. 1,3-Propanediamines substituted at the 1 and/or 3 positions can be obtained from the corresponding 1,3-dicarbonyl compound by conversion to the dioxime followed by reduction to the diamine. This general method is also applicable to 1,2,3-trisubstituted-1,3-propanediamines by starting with the appropriate 2-substituted-1,3-dicarbonyl compound.

Transition metal complexes of the present invention can be made as described herein. The following specific ligands can be reacted with transition metals to form TMC which are useful in lowering and controlling IOP. ##STR2##

The anterior and posterior chambers of the eye are filled with aqueous humor. During ophthalmic surgical procedures, such as the removal of cataracts, which require incisions which expose the anterior and/or posterior chambers to the environment, it is usually necessary to fill the eye with a material such as exogenous sodium hyaluronate, Healon® (Pharmacia Ophthalmics, Pasadena, Calif.). These materials, among other things, help prevent the eye from collapsing and provide, to the otherwise exposed tissues, some protection from the environment. Sodium hyaluronate is typically the material used for the above-stated purposes. However, while its instillation into the eye has its benefits, a problem associated with its use is that following surgery the IOP of the eye is significantly raised above normal levels until the sodium hyaluronate is diluted and flushed from the eye through the trabecular meshwork and extracellular matrix due to the natural production and turnover of aqueous humor.

The present invention provides for the lowering of such raised IOP by administering the TMC disclosed herein to the open eye containing the sodium hyaluronate just prior to closing of any incisions. While not wishing to be bound by any theory it is believed that the TMC lower IOP caused by the instillation of sodium hyaluronate by aiding in the substantially irreversible oxidative breakdown of the sodium hyaluronate into fragments which are more readily washed from the eye via the extracellular matrix.

The transition metal complexes of the present invention may be incorporated in various formulations for delivery to the eye. For example, topical formulations can be used and can include ophthalmologically acceptable preservatives, surfactants, viscosity enhancers, buffers, sodium chloride and water to form aqueous sterile opthalmic solutions and suspensions. In order to prepare sterile ophthalmic ointment formulations, a transition metal complex is combined with a preservative in an appropriate vehicle, such as mineral oil, liquid lanolin or white petrolatum. Sterile ophthalmic gel formulations comprising TMC can be prepared by suspending the TMC in a hydrophilic base prepared from a combination of, for example, Carbopol-940 (a carboxyvinyl polymer available from B. F. Goodrich Company) according to published formulations for analogous preparations. Preservatives and tonicity agents may also be incorporated in such gel formulations.

Topical ophthalmic aqueous solutions, suspensions and gels of TMC of ligands [1], [2], [3] and [4] are preferred dosage forms. Topical formulations of the Fe⁺³ complex of ligand [4] is particularly preferred. The TMC will normally be contained in the formulation at a concentration between about 0.2 and 6.0 weight percent (wt. %), preferably 2.0 to 4.0 wt. %, most preferably about 2.0 wt. %. The compounds are administered by instillation of 10-70 ul, preferably about 40 ul of a TMC solution to the open eye, in which sodium hyaluronate has been used during surgery, prior to the closing of any incisions. Instillation of about 40 ul of a 2% solution is preferred.

The following examples illustrate the preparation of some specific TMC and formulations comprising the complexes which can be used according to the present invention for the lowering and control of elevated intraocular pressure resulting from the instillation to the eye of exogenous sodium hyaluronate.

EXAMPLE 1 Preparation of N,N'-(1,3-Propanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt

The iron complex, sodium salt of ligand [2] was prepared from commercially available propylenediaminetetraacetic acid (Aldrich Chemical Company) using the general method described in Sawyer et al., J. Amer. Chem. Soc., Vol. 82, p. 4191 (1960).

EXAMPLE 2 Preparation of N,N'-(trans-1,2-Cyclohexanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt

The iron complex, sodium salt of ligand [3] was prepared from commercially available trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid hydrate (Aldrich Chemical Company) as described below.

To a suspension of trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid hydrate (10 g, 28.9 mmol) in 0.5M aqueous ferric chloride (57.8 ml, 1 eq) was added portionwise sodium bicarbonate until the pH was 5.5. The mixture was refluxed for 20 min, cooled, filtered through celite, and evaporated. The residue was taken up in warm (70° C.) dimethylsulfoxide (200 mL) and filtered to remove inorganic salts. After the dimethylsulfoxide was removed by evaporation, the residue was recrystallized from water/ethanol to provide 5.5 g (45%) of the complex as green crystals.

Anal. Calcd. for C₁₄ H₁₈ N₂ O₈ FeNa 2H₂ O: C, 36.78; H, 4.85; N, 6.12 Found: C, 36.27; H, 4.90; N, 6.12.

EXAMPLE 3 Preparation of N,N'-(1,2-Propanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt

The iron complex, sodium salt of ligand [4] was prepared from commercially available 1,2-diaminopropane-N,N,N',N'-tetraacetic acid (Aldrich Chemical Company) as described below.

1,2-Diaminopropane-N,N,N',N'-tetraacetic acid (10.0 g, 32.6 mmol) was treated with 1 eq of 0.5M aqueous ferric chloride as described in Example 2. Recrystallization from water/ethanol provided 11.2 g (90%) of the complex as a yellow solid.

Anal. Calcd. for C₁₁ H₁₄ N₂ O₈ FeNa 1.5H₂ O: C, 32.37; H, 4.20; N, 6.86. Found: C, 32.17; H, 4.26; N, 6.78.

EXAMPLE 4 Preparation of 2,2'-[(1,2-Ethanediyl)bis[(carboxymethyl)-imino]] bispropionic acid, iron complex, sodium salt

The iron complex, sodium salt of ligand [5] was prepared from commercially available ethylenediamine-N,N'-diacetic acid-N,N'-di-α-propionic acid (Sigma Chemical Company) as described below.

Ethylenediamine-N,N'-diacetic acid-N,N'-di-α-propionic acid (5.0 g, 15.6 mmol) was treated with 1 eq of 0.5M aqueous ferric chloride as described in Example 2. Recrystallization from water/ethanol provided 1.2 g (19%) of the complex as a bright yellow solid.

Anal. Calcd. for C₁₂ H₁₆ N₂ O₈ FeNa 2H₂ O: C, 33.43; H, 4.66; N, 6.50. Found: C, 33.58; H, 4.63; N, 6.47.

EXAMPLE 5

The following aqueous formulation can be used to lower and control elevated intraocular pressure resulting from the instillation of exogenous sodium hyaluronate to the eye during surgery. The formulation can be administered topically to the open eye prior to closing any incisions.

    ______________________________________                                         Ingredient           Concentration (w/v %)                                     ______________________________________                                         N,N'-(1,2-Propanediyl)bis[N-(carboxy-                                                                2.04                                                     methyl)glycine],                                                               iron complex (Ligand 4 complex)                                                Mannitol             3.6                                                       Purified water       q.s. to 100 ml                                            ______________________________________                                    

Procedure

Approximately 85% (8.5 ml) of the batch volume of purified water was added to a container. 204 mg of Ligand 4 complex and 360 mg of mannitol were added to the container and mixed well. The pH was adjusted to 6.0 with 0.01N NaOH. The solution was then filtered through a sterilizing filter into a sterile receiving vessel. Purified water (q.s. to 10 ml) was poured through the sterilizing filter and the solution was mixed well.

EXAMPLE 6

The following gel formulation can be used according to the methods of this invention to lower and control elevated intraocular pressure resulting from the instillation of exogenous sodium hyaluronate into the eye.

    ______________________________________                                         Ingredient         Concentration (w/v %)                                       ______________________________________                                         Carbopol-940 (B. F. Goodrich                                                                      3.0                                                         Company)                                                                       Mannitol           3.6                                                         Benzalkonium chloride (BAC)                                                                        0.01                                                       Ligand 1 Complex   2.0                                                         Purified water     q.s. to 100%                                                ______________________________________                                    

Procedure

Place approximately 85% (8.5 ml) of the batch volume of purified water in a container. Add all of the ingredients to the container: 0.36 g mannitol; 0.2 g Ligand 1 complex; 0.1 ml of 1% BAC; and 0.3 g carbopol and mix well. The pH is adjusted to 6.5 with 0.01N NaOH. Purified water (q.s. to 10 ml) is then added and mixed well to form a gel. 

We claim:
 1. A method for lowering elevated intraocular pressure resulting from the instillation of exogenous sodium hyaluronate to the eye during surgery, which comprises administering topically to the eye a therapeutically effective amount of a composition comprising a compound selected from the group consisting of N,N'-(1,2-ethanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt; N,N'-(1,3-propanediyl)bis-[N-(carboxymethyl)glycine] iron complex, sodium salt; N,N'-(trans-1,2-cyclohexanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt; N,N'-(1,2-propanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt; 2,2'-[(1,2-ethanediyl)bis[(carboxymethyl)imino]] bispropionic acid, iron complex, sodium salt; N,N'-(2,2-dimethyl-1,3-propanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt; N,N'-(2-ethyl-1,3-propanediyl)bis[N-(carboxymethyl)glycine, iron complex, sodium salt; and N,N'-(2-methyl-1,3-propanediyl)bis[N-(carboxymethyl)glycine], iron complex, sodium salt.
 2. The method of claim 1 wherein the compound comprises N,N'-(1,2-propanediyl)bis[N-(carboxymethyl)glycine] iron complex sodium salt.
 3. The method of claim 1 wherein compound concentration is between about 0.2 and 6.0 weight percent.
 4. The method of claim 3 wherein the concentration is between about 2.0 and 4.0 weight percent. 